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These sequences were analyzed for intron/exon boundaries, as well as missing or incomplete exons that varied from known gene structure, by conducting multiple sequence alignments of protein sequence deduced from the Hessian fly genome with deduced protein sequences obtained from orthologs of other insect species.
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Next, MSA Generator conducts multiple sequence alignment for individual gene families using MAFFT 7.0 [ 18].
The conservative motifs among AP2/ERF proteins in plants were clarified by conducting multiple alignment analyses using amino acid sequences of the AP2/ERF domain.
We conducted multiple-sequence alignment using MUSCLE (4 ) and optimized the sum of all of the pairs of characters manually.
An out-of-autoclave unidirectional prepreg system by SOLVAY (CYTEC), CYCOM 5320/T650-35 was used to conduct multiple forming experiments and study the effects of various parameters including forming temperate and lay-up sequence.
Phylogenetic analysis was conducted by carrying out multiple sequence alignments using the ClustalW online tool (available at http://www.ebi.ac.uk/Tools/msa/clustalw2/) for the protein sequences using default settings.
By conducting a manual pairwise sequence comparison, one reference sequence was responsible for this result.
In order to identify potential functional motifs of MazF proteins, phylogentic analyses of MazF proteins were conducted by applying the embedded multiple sequence alignment ClustalW program in the Geneious software package v 5.5.7 [ 76, 77] with the Neighbor Joining method.
A significant improvement is obtained by combining multiple sequence alignments with the GOR method.
We conducted sequence-based analyses to identify these amino acid residues by performing a multiple sequence alignment and using the AVANA tool (http://sourceforge.net/projects/avana/) to compare the mutual information between subsets of the alignment for the location of the characteristic sites [17].
This is followed by multiple sequence alignment.
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