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For some genera, a chimaeric sequence was formed by concatenating sequences from two congeneric species (Additional file 6).
For GMAP, we determined CDS ranges by concatenating sequences from proposed exons and then used this transcript model as the query in a BLASTx [ 23] search against human proteins.
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As a result, in Crataegus a reasonable degree of phylogenetic resolution was only obtained by concatenating sequence data from 14 plastid loci (Fig. 1; cf. Lo and Donoghue 2012), but this is not a solution acceptable from the current barcoding point of view.
We then concatenated sequences from nine files and transferred to a directory for analysis using mothur software [47].
The phylogenetic analyses were performed on both the separate, and concatenated, sequences from the E1/E2 and NS5B genes.
For the phylogenetic analysis, we concatenated sequences from the 9 studied spacers.
We concatenated sequences from the Ef-2, RPB1, RPB2 and actin loci for maximum likelihood and Bayesian analyses.
Relatively well-resolved phylogenetic analyses required concatenated sequences from a total of 14 plastid loci (Fig. 1).
Thus, the tandem concatenated sequences from three motifs were selected to serve as the indicators selecting the substrates.
This sequence was generated by concatenating partial sequences from six different ILTV strains.
To analyze differences between the genome and transcriptome, we constructed genome-based predicted transcripts by concatenating genomic sequences from GRCh37 according to exon boundaries posted by the UCSC genome browser.
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