The off line analysis was performed using a computer based image analysis program (CapImage®, Engineering Office Dr. Zeintl, Heidelberg, Germany).
P63 staining was visually assessed by the same physician who at the same time marked regions on the substrate of the slide, indicating regions of interest for subsequent computer based image analysis.
The number of colonies was automatically quantified by computer based image software (Image J).
These drawings were scanned, digitalized, and the area of the sections and of the alpha or beta cell clusters immunostained for glucagon or insulin was measured by a computer based imaging analysis system (Scion Image; Scion Corporation).
The collapsed elastic tube shape is determined by computer tomography based image analysis and velocity profiles are monitored using pulsed ultrasound Doppler velocimetry technique.
Object based image analysis.
Stained sections were quantitatively evaluated by computer-based image analysis, and results were expressed as the percentage of positive stained area versus total tissue staining.
An alternative approach is to quantify PMD by using computer-based image-analysis techniques of mammograms [ 7- 11].
The density of air-exchanging parenchyma, A A (ae/lu), was determined by point counting based on computer-assisted image analysis.
However, the network of blood vessels was less complex than the one induced by recombinant VEGF, as assessed by our original method of vessel quantification based on computer-assisted image analysis (Blacher et al, 2001).
The18 HPF images are analyzed by computer-assisted image analysis using a Syndia algorithm on a Qwin-based analysis system (Leica, Cambridge, UK) (Haringman et al. 2005).
