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Quantitative determination of density of the [S]klk1b26 protein and [S]GAPDH protein bands was performed by computer assisted image analysis of the autoradiograms.
Histological features were assessed on samples taken from 31 subjects at baseline and week 24, and skin surface profilometry replicas of the crow's feet area were taken from 53 subjects (8 or 9 per group) pre- and post-treatment and assessed by computer assisted image analysis.
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Quantification was performed by raster electron microscopy and computer assisted image analysis.
The number of cells was estimated by optical fractionator probe using a computer assisted image analysis system with a Zeiss Axioplan 2 microscope connected to a digital camera.
Using zymography and computer assisted image analysis, we have measured the levels of type IV collagenases in biopsies from normal breast, and benign and malignant breast disease.
Total lesion area or area of positive staining was determined for each section/aorta, respectively, using computer assisted image analysis NIS-elements/Lucia GG software, Nikon UK).
The slices were photographed and infarct size was calculated using automatic computer assisted image analysis software (Clemex, Quebec, Canada) as described before [ 29].
The animals were infused with 0.5 mg/kg LPS (E. coli O55 B5) over 80 min. Vascular leakage was detected with FITC-marked rat serum albumin by fluorescence microscopy and evaluated by grey value analysis with a computer assisted image processing system.
The positive area of each glomerulus was measured by computer-assisted image analysis (KS400, Zeiss, Germany).
The intensity of the radioactive bands was evaluated by computer-assisted image analysis.
The18 HPF images are analyzed by computer-assisted image analysis using a Syndia algorithm on a Qwin-based analysis system (Leica, Cambridge, UK) (Haringman et al. 2005).
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