Sentence examples for by computational mutation and from inspiring English sources

The atomic coordinates of the AKAP PKA binding helix in complex with the RIIα docking and dimerization domain (RIID/D-AKAPhelix; PDB 2IZX) (Gold et al., 2006) was used as a template to model the corresponding region in AKAP18γ, residues 301 317, by computational mutation and minimization in COOT (Emsley and Cowtan, 2004).

Although we could estimate the effect of the variance of RP1L1 gene by computational mutation analysis and their amino acid conservation in other species, we need to confirm the missense effect by their functional aspect, for example, making knock-in mice with the mutation, and protein interaction assay with mutant proteins.

As the pace of variant discovery currently far outstrips functional characterization, analyses will likely continue to focus on non-synonymous variants classified by computational mutation prediction algorithms.

For these 40 residues, we performed computational mutation analysis by substituting each of the interface receptor residues by the remaining standard amino acids.

We develop a computational approach to mutation and selection in a changing environment displaying NFDS.

This list was recently extended by 11 disease-related genes, where uORF-altering mutations were identified by computational analysis of the Human Gene Mutation Database 18. Diseases with confirmed uORF mutations include the van der Woude syndrome (IRF6), hereditary pancreatitis (SPINK1), and familial hypercholesterolemia (LDLR) 18.

This distribution is changed by mutation and cross-over.

All these considerations point to the conclusion that significantly better tools are needed for a quantitative analysis of mutations by computational means.

Molecular dynamic simulation (MDS) had been a preferred platform to study the phenotypic effect induced by point mutations by computational biologists [ 60, 61].

Interactions inferred by computational methods (37, 38) and genetic interactions, which represent phenotype alterations produced by the mutation/deletion of one gene in the background of a mutation/deletion of another gene (39 41), were not considered here mainly because only a small subset of the databases curate them.

Five optimal single mutants were predicted by the computational design, and the N52Y mutation showed the thermostabilization effect.