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Messenger RNAs (mRNAs) predicted to be target of hsa-miR-9* were identified by computational analysis, using web-available resources (Mirnaviewer, PicTar, Tarbase [21] and miRBase [22]; mirnaviewer is available at http://cbio.mskcc.org/mirnaviewer; PicTar is a project of the Rajewsky lab at NYU's Center for Comparative Functional Genomics and the Max Delbruck Centrum, Berlin).
RNA binding residues in CENP-A, H3, Scm3, and HJURP were predicted by computational analysis using the BindN+ program.
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Predicted bovine NCBI transcripts are generally derived by automated computational analysis using the NCBI gene prediction method GNOMON (http://www.ncbi.nlm.nih.gov/genome/guide/gnomon.shtml).shtml
A fragment of the FBXW7 3′-untranslated region contained three putative miR-223 binding sites as determined by a computational analysis using miRNA target prediction programs such as Target Scan (http://www.targetscan.org) and miRanda (http://www.microrna.org) (Supplementary Figure 1).
The purified intact protein (100 pmol) was analyzed by LC MS, and computational analysis using the averagine model could detect the dibromide-labeled from the unlabeled protein (Supplementary Figure 8).
In addition to observing the RpoN regulon by using gene expression microarrays, we employed computational analysis using the PromScan software [ 27] to identify RpoN recognition sequence elements within the G. sulfurreducens genome.
Computational analysis using NSITE software (Softberry Inc).
To further analyze the mechanism, we performed computational analysis using ordinary differential equations (ODEs) model.
Domains were predicted by computational analysis of this protein sequence using the SMART bioinformatics tool package (Fig. 3) [55].
Microarray expression profiling was performed using Illumina mouse-6 v1 beadchips, followed by computational analysis.
Thus, the experimental analysis using RT-PCR and northern blotting demonstrate that the snoRNA predictions by computational analysis are indeed valid and correspond to authentic snoRNA genes.
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