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Several manufacturing options have been investigated and were evaluated by computational analysis and fabrication of pre-prototypes.
The physicochemical and biological properties of this peptide were evaluated by computational analysis and its isoelectric point, grand average of hydropathicity and Boman index values were found to be 6.74, 0.42 and −1.17 kcal/mol, respectively.
We identified putative ETS binding sites (EBSs) within 1 kb of the EZH2 TSS by computational analysis and performed ChIP experiments to determine whether ERG was able to bind to these sites (Fig. 3F).
The identified proteins are contrasted by computational analysis and their sequences are inspected to correct possible errors in function prediction.
In this study, we evaluated a literature-based dynamic metabolic pathway model of E. coli by computational analysis and verified it experimentally.
Among these bHLH members, 34 hypothetical proteins, such as LOC100124777, were newly annotated by computational analysis and verified by EST searching in this research.
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The Giardia U5-snRNA was identified by computational analysis [8], and it folds into a conserved U5 secondary structure, although the primary sequence itself does not show homology with U5-snRNAs from other species.
Contiguous domains of open and closed chromatin were identified by computational analysis of the data, and correlated to other genome annotations including predicted chromatin "states", individual chromatin modifications, nuclear lamina interactions, and gene expression.
Thus, the experimental analysis using RT-PCR and northern blotting demonstrate that the snoRNA predictions by computational analysis are indeed valid and correspond to authentic snoRNA genes.
The resulting double mutants were initially grown on standard media and the fitness of each double mutant colony assessed by computational analysis of colony size and classified according color-coded categories of deviation from the median colony size following the criteria (see supplementary Fig. 5).
Recently the design of a novel in vitro immune diagnostic enzyme-linked immunospot (ELISPOT) and whole blood ELISA (WBE) for IFN-γ using multiepitopic peptides that are selected by computational analysis from CFP-10 and ESAT-6 as stimulating antigens has been reported [14].
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