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As ABT-737 is acting by complex disruption between pro- and anti-apoptotic proteins, we next investigated some complex disruptions by co-immunoprecipitation in PC-3, HT-29 and Jurkat mitochondria treated with ABT-737 (Fig. 7).
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The interference model of activator-αCTD complex disruption by Spx is consistent with the observation that free Spx does not bind to DNA [10].
WB analysis showed that complex disruption induced the DNA damage shown by increased γH2AX staining by WB and flow cytometry.
All the kinetic parameters involved in the ligand oxidation and in the complex disruption were calculated on the basis of the agreement between experimental and simulated linear sweep and cyclic voltammetries.
As in the Fas DD/FADD DD complex, disruption of the type IIa interface by the N150K mutation did not show a distinct effect on complex formation.
In addition to identifying disruptions of terminal steps, we also identify complex disruption combinations in upstream pathways that indirectly negate the targeted output by propagating their action through the signaling cascades.
The financial services industry, long protected by complex regulations, high barriers to entry and economies of scale, is ripe for disruption.
Rail passengers, already hit by strike action and engineering works, have experienced "significant disruption" between Penzance and Bristol.
Cardiogenesis is regulated by a complex interplay between transcription factors.
More recently, Gao et al. argue that the mechanism of transcriptional repression by VENTX is caused by the disruption of the complex between β-catenin and TCF/LEF factors [48].
Such localization of excessive glycolipid binding GLTP-folds could impact cellular processes involving more complex GSLs by homeostatic disruption of GSL metabolism.
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