Recently we reported that intratumoral injection of a short CAP, LTX-302, derived from the naturally occurring CAP bovine lactoferricin (LfcinB), leads to a local inflammation followed by a complete regression of the tumor.
A single AC injection in mice with a medium tumour volume of 100 mm (usually 75 days after tumour graft) induces a complete regression of all tumours by day 35, both by local palpation and histological analysis of the injected fat pads Figure 2A.
All patients in the supplement group reported complete regression of symptoms by the end of the study, compared with only 66% in the drug-treated group.
We have also demonstrated the potential of the PCI technology in vivo in an animal model, where the cytotoxic effect of gelonin, which was completely inactive when used alone, was increased tremendously by photochemical treatment, resulting in a complete regression of subcutaneous tumours in mice (Selbo et al, 2001).
To confirm complete regression of the tumour by histopathology, biopsies were taken after treatment.
In addition to early onset of gastrointestinal tumors, ApcMin mice show progressive loss of immature and mature thymocytes from ∼80 days of age, with complete regression of the thymus by 120 days [28].
In mouse models, wherein metastatic mammary carcinomas are induced by the doxycycline induction of Neu overexpression, withdrawal of the Neu oncogene by cessation of doxycycline therapy results in complete regression of the mammary tumours and its associated tumour metastases (Moody et al, 2002).
Daayana et al (2010) further showed in a paper published in British Journal of Cancer that Imiquimod topical application followed by HPV therapeutic vaccine led to the complete regression of VIN in 63% of patients recruited in a Phase II clinical trial at 52 weeks after the treatment.
Professional processing and presentation of tumour antigens by DC elicits anti-tumour reactivity and causes complete regression of established NB tumours.
