Sentence examples for by complementation with the from inspiring English sources

Arabidopsis mutants lacking AHK5 show reduced stomatal closure in response to H2O2, which is reversed by complementation with the wild type gene.

Both defects were corrected by complementation with the wild-type allele (15).

This was further confirmed by complementation with the zur gene in trans, which restored the expression pattern observed in the wild type.

For microscopy analyses of colony spatial organisation, BY4741 had prototrophy restored by complementation with the above fluorescent protein vectors (Table 1).

A micro-colony of BY4741 with prototrophy restored by complementation with the fluorescent protein vectors yEpCFP_HIS (HIS3), yEpSapphire_LEU (LEU2), yEpVenus_URA (URA3) (Bilsland et al., 2013), and pRS411- GPDpr-mCherry (MET15) (Table 1) was grown for 2 nights on SM.

We confirmed that the transformant YPH500m4 was met4− by complementation with the MET4-bearing plasmid pYCM4 (provided by Dr. Omura, Institute for Liquor Products, Suntory Ltd .. Saccharomyces cerevisiae SF1-1C was grown overnight in YPD and then transferred to either SF or fresh YPD media.

The mutant phenotype could be reverted by complementation with a plasmid carrying the coding region of arsR, demonstrating that the observed phenotype was due to arsR.

The synthetic growth defect exhibited by the interaction between myo1Δ and gcn2Δ mutations was rescued by complementation with a plasmid copy of the wild-type MYO1 gene, myo1Δgcn2ΔpRS316-MYO1 + indicating that it was not caused by secondary mutation(s).

As also reported previously [15], expression of Ssp4 by DS cultures of SM101::ssp4 was restored by complementation with a shuttle plasmid carrying the wild-type ssp4 gene.

The strain Msm uvrB: puvrBMtb was generated by deleting M. smegmatis uvrB by homologous recombination with selection for hygromycin resistance, followed by complementation with uvrB from Mtb on the integrative plasmid pMV306 behind a constitutive hsp60 promoter.

Colony formation was restored in Smyd3 depleted cells by complementation with wild-type Smyd3 (lacking the 3′ UTR and therefore RNAi-resistant) (Figs. 3A and B), whereas complementation with catalytically dead Smyd3 (Smyd3N205A (Fig. 3A) and Smyd3F183A (Fig. 3B) failed to reconstitute this activity.