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The virus was quickly recognized as an influenza A virus by complement fixation tests.
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A panel of 180 horse field sera, previously sent for testing to the German National Reference Laboratory for Dourine, was tested by complement fixation test using culture-derived as well as conventionally produced dourine antigen.
Antigens of respiratory syncytial virus (RSV), adenoviruses, influenza A and B, and parainfluenza types 1 3 in NPAs and MEFs were detected by time-resolved fluoroimmunoassay (TR-FIA), and antibody titers were determined by complement fixation test (CFT) or by enzyme immunoassay.
The virus was identified by complement fixation test and confirmed by reverse transcription polymerase chain reaction.
Furthermore, antibodies to CHLV and POTV cannot be differentiated by complement fixation test (4 ).
Identification of isolates was performed by complement fixation test as reported (9 ).
Serum antibodies were analysed by ELISA and positive samples were retested by Complement Fixation test (CFT) to detect recent infections.
These samples were analysed by the Rose Bengal Plate test (RBPT) test, with all positive samples being confirmed by complement fixation test (CFT).
Serotyping of FMDV was carried out by complement fixation test at NVI (13 ) and by antigen-detection ELISA at WRLFMD (15 ).
The antibody titre against influenza A by complement fixation test increased from 80 on admission (7 days after onset of symptoms) to 2560 on day 5 after admission.
The Rose Bengal Plate Test (RBPT) was conducted according to manufacturer's manual (Prionics AG, Schlieren-Zurich, Switzerland) and samples positive by RBPT were subsequently tested by complement fixation test (CFT).
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