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A panel of 180 horse field sera, previously sent for testing to the German National Reference Laboratory for Dourine, was tested by complement fixation test using culture-derived as well as conventionally produced dourine antigen.
Antigens of respiratory syncytial virus (RSV), adenoviruses, influenza A and B, and parainfluenza types 1 3 in NPAs and MEFs were detected by time-resolved fluoroimmunoassay (TR-FIA), and antibody titers were determined by complement fixation test (CFT) or by enzyme immunoassay.
The virus was identified by complement fixation test and confirmed by reverse transcription polymerase chain reaction.
Furthermore, antibodies to CHLV and POTV cannot be differentiated by complement fixation test (4 ).
Identification of isolates was performed by complement fixation test as reported (9 ).
Serum antibodies were analysed by ELISA and positive samples were retested by Complement Fixation test (CFT) to detect recent infections.
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The virus was quickly recognized as an influenza A virus by complement fixation tests.
All sera sent to the National Reference Laboratory for Toxoplasmosis in the National Institute of Public Health were examined by complement-fixation test (CFT) using a commercial CFT kit (SEVAC, Prague), by IgG ELISA (SEVAC, Prague), and by IgM ELISA (TestLine, Brno).
Antibodies against C. burnetii are usually detected by enzyme-linked immunosorbent assays (ELISAs), indirect immunofluorescence (IFA) or by the complement fixation test (CFT).
All animals showed a specific antibody response detected by the complement fixation test (CFT) (data not shown).
The diagnosis of infection was performed by the complement fixation test, molecular methods (PCR), and, in some cases, indirect haemagglutination and xenodiagnosis.
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