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Before one can understand the abnormal conditions, it is valuable to understand the scope of normal alternative splicing by comparing splicing patterns between normal tissues.
Statistical significance was determined by comparing splicing patterns in treated cells to untreated cells with a two-tailed Student's t-test (n ≥ 2).
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By comparing splice events for smokers and nonsmokers, we were able to demonstrate there are no overall smoking-dependent changes in the patterns of splicing for either ubiquitous or SAE-enriched genes.
The effect of smoking on alternative splicing was estimated by comparing normalized splice junction usage.
In the case of 395 W1943 FLcDNAs that were 100% matched to the genomic sequences, we checked the splicing patterns by comparing with all rice ESTs or mRNAs in public databases.
The orthology between X. tropicalis and human ADAMs were further supported by conserved splicing patterns and synteny.
Moreover, it may be used for cellular phenotyping and help establishing the etiology of diseases characterized by abnormal splicing patterns.
Nonetheless, alternative splicing could be contributing to the lack of overall gene expression change if underpinned by changes in splicing patterns during myogenesis.
To compare mouse and human splicing patterns, we identified human alternative exon trios orthologous to mouse alternative and flanking exon trios using synteny in a multi-genome alignment.
We searched for cases of alternative splicing by comparing transcripts defined by the Cufflinks program.
In each case, we observed that the mutation dramatically increases splicing at the alternate splice site when compared with the splicing pattern of RNA extracted from control keratinocytes.
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