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By comparing sequencing of the transcriptome (RNA-seq) with the genome, the authors were able to identify alterations in two transcripts (COG3 and SRP9) that result in variant protein sequences not encoded by the genomic tumour DNA, suggesting that these variants were caused by RNA editing.
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Taxonomies were assigned by comparing sequences of contigs and singletons to the entire NCBI nucleic acid and protein databases.
It works by the logic that, if groups B and C have more similarities to each other than either has to group A, then B and C are more closely related to each other than either is to A. Characters that are compared may be anatomical, such as the presence of a notochord, or molecular, by comparing sequences of DNA or proteins.
The minimum interspecific distances were calculated by comparing sequences of the species of interest to sequences of all remaining species in the dataset.
Two non-reference alleles of Myo15 were identified by comparing sequences of the mutated rat strain LEW/Ztm-ci2 and its co-isogenic background strain LEW/Ztm.
PCR products were cloned into EcoRV digested pBluescript and sequence integrity confirmed by comparing sequences of four individual clones from separate PCR reactions with the sequence for Brown Norway rat (Ensembl, ENSRNOG00000031367).
The diversity of Pseudomonas species present between the two patient groups was analysed by comparing sequences of the 617 bp P16S PCR products from 13 CD and 12 non-IBD patients.
Characterization of novel rickettsial species was achieved by comparing sequences of genes as described (9 ).
The two sequences of a heterozygote were separated by comparing sequences of the PCR product and the cloned sequences.
The two sequences of a heterozygote were separated by comparing sequences of the PCR product and cloned sequence.
Multilocus sequence typing (MLST) is a method for characterization of bacterial isolates by comparing sequences of several gene fragments.
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CEO of Professional Science Editing for Scientists @ prosciediting.com