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The position and orientation of each Gal gene were obtained by comparing its cDNA with the assembled DNA sequence.
The relative positions, orientations, and structural organizations of individual genes were determined by comparing its cDNA sequence to the continuous genomic contig that we assembled.
The exon/intron organization of the gene encoding Ci-Syn was deduced by comparing its cDNA sequence with the genomic sequences of scaffold 53 (release version 1.0) and chromosome 5 (release version 2.0) (Additional file 1).
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In order to measure whether a given gene was significantly expressed, we compared its cDNA's signal to a signal distribution derived from negative controls represented by approx. 2,500 empty spot positions on the array.
Thus, relative expression level of each OR-coding sequence may be roughly estimated by comparing its coverage number in cDNA-originated OR sequences with that in genomic DNA originated sequences, provided that similar numbers of OR-coding reads are obtained from the two sources (table 2).
It is possible that polymorphisms may be found by comparing the cDNA sequences to the genome, because our cDNA sequences were derived from M. oryzae strain KJ201, not strain 70-15, wasch was used for genome sequencing; however, on average, the two strains are more than 99% identical in exon regions.
By comparing the ASAP cDNA and its deduced protein sequence with the human genome and the putative transcripts of the Ensembl database, we assigned the ASAP gene to chromosome 4q32.1.
Genomic organization and chromosomal locations were investigated by comparing the cDNA and corresponding genomic sequence (http://genome.ucsc.edu/).ucsc.edu/
Brain-specific and ovary-specific A-to-I RNA editing sites were further verified by comparing the cDNA sequences with their corresponding genomic templates in multiple cell lines from brain, colon, breast, bone marrow, lymph, liver, ovary and kidney tissue.
The exon structure was confirmed by comparing the cDNA with nucleotide contig NT_009866.
RNA editing sites were analyzed by comparing each cDNA sequence to that of genomic DNA.
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CEO of Professional Science Editing for Scientists @ prosciediting.com