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However, verification of the probable epithelial origin of differentially expressed genes can be obtained by comparing expression data from breast epithelial cell lines [ 22], breast tumour cell lines or, as in the present study, by immunohistochemistry, all of which show that, for example, IL8, is a bona fide epithelial tumour-associated product [ 43, 44].
The reproducibility and robustness of the molecular scores was validated by comparing expression data with RNA from FF and FFPE material of 82 tumors.
- The hook method performs reasonably well by comparing expression data of the same origin between two chip generations (HG-U133A and HG-U133 plus 2.0).
We hypothesized that miRNAs would play a role suppressing certain genes during hypoxia, and tested this by comparing expression data from miRNA and mRNA expression profiles investigated in parallel.
By comparing expression data between amplified and non-amplified material, we have observed that the ratios were significantly different for a number of genes, in addition to a set of genes whose ratios were neither conserved nor rejected.
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Previous studies have identified genes important to the stem cell state by comparing expression profiling data from different stem cell populations such as ES cells, haematopoietic stem cells (HSCs) and neural stem cells [ 6, 18].
An alternate way of analyzing the same data is by comparing expression levels at a given time point to those previous to the shock (Additional file 1: Figure S2).
A control for experimental and biological variance was conducted by comparing mouse expression data from Novartis [ 17] with and independently obtained mouse expression data set [ 41].
The existence of population-specific differences in expression levels of probes was determined by comparing the expression data using a Kruskal-Wallis test.
More detail analysis by comparing the expression data with phylogenetic relationship showed that expression divergence was observed even in closely related genes.
The set of genes that show a predominant expression in the tracheal epithelium was identified by comparing the expression data from the airway epithelium with those of the remainders of the animals.
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