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These tRNAs have cloverleaf secondary structures that were derived by comparative analysis using an alignment that is most compatible with tRNA phylogenies and known 3-dimensional models of structure [91], [92].
The plasmids were analyzed by comparative analysis using MAUVE algorithm (Darling et al. 2010) and with the comparative analysis tools of RAST (Aziz et al. 2008).
Approximately half of the putative genes in S. lineatus and 60% in S. mimosarum and S. tentoriicola were annotated by comparative analysis using BLASTx against the NCBI non-redundant protein database (see Table 1).
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The chloroplastic localization of the vd-5'UTR/GFP was confirmed by a comparative analysis using a GFP fused to a chloroplast-specific transit peptide derived of a protein of the oxygen evolving complex (OE23) as reference [20].
To assess whether KN400 was a particularly fortuitous choice for attempting a de novo assembly using only short reads, we assessed its genome complexity by performing a comparative analysis using five different genomic properties that have been characterized using 895 microbial genomes.
NHEJ has been suggested to associate with chloroplast DNA insertion into nuclear genome (Lloyd and Timmis 2011), and this is supported by the current comparative analysis using Oryza genome sequence data.
Furthermore, to confirm whether our full-length cDNAs contain genes expressed in specific conditions, a comparative analysis using genes regulated by osmotic stress and ABA treatment was performed.
Viral loads were estimated by comparative analysis by using threshold values obtained with serial dilutions of a 450-nucleotide (nt) in vitro transcribed RNA, quantified spectrophotometrically as previously reported (21 ), encompassing the target region (detailed protocol available on request).
To elucidate a mechanism of Bcl11b regulation of neuronal differentiation and progenitor proliferation, we determined target genes by comparative transcriptome analysis using RNA of dentate gyrus tissue harvested from control and mutant animals at P14.
Using this system, we then compared the proteomes of cells expressing shWRN for 3 and 5 days with those of control cells expressing shRNAs against the green fluorescence protein (GFP) (shCTR) for 5 days by two-dimensional difference gel electrophoresis (2D DIGE) (Marouga et al., 2005) followed by comparative analysis of all spots using DeCyder differential in-gel analysis (DIA) software.
These observations were bolstered by the comparative GO analysis using the extensive EST collection generated for the desiccation sensitive moss Physcomitrella patens.
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