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Routine liver and renal function was measured by commercially available methods.
Neutrophil gelatinase-associated lipocalin (NGAL) has emerged as the most promising biomarker of kidney injury; this protein can be measured by commercially available methods in whole blood, plasma, serum, and urine.
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The DEQAS has shown that samples analyzed by most commercially available methods are capable of providing results close to the target value for 25(OH)D3, but that results are highly dependent on the laboratory of analysis and the skill of the operator.
By contract, the SRLs all use commercially available methods, identical to those used in clinical laboratories.
(commercially available methods).
Circulating plasma levels of adiponectin, fetuin-A, and TNF- α were measured by a commercially available method, using Quantikine reagents (R&D Systems, Minneapolis, USA).
Sepsityper (Bruker Daltonics) allows the easy and fast identification of bacteria from positive blood cultures by using MALDI-TOF MS. Another commercially available method, MolYsis (Molzym), is capable of removing human background (cells and DNA) from blood (culture) samples by selective lysis and, additionally, ensures pathogen enrichment.
Malonic dialdehyde (MDA) determination was done by HPLC according to the method of Wong et al Neopterin (NPT), Elastase, Procalcitonin (PCT) and antibodies to oxidised LDL (oLAb) were determined by commercially available ELISA methods.
Microrods were fabricated photolithographically by commercially available materials using methods adapted from previously designed protocols by our group and others, and are shown schematically in Figure 1B.
Level of free 8-Isoprostane was assessed by commercially available enzyme immunoassay (EIA) method.
Further minimization of contamination during pre-sequencing PCR reactions could be achieved by commercially available uracil-N-glycosylase-based methods designed to degrade amplicons which may inadvertently be present in samples before PCR amplification.
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