Sentence examples for by combining two primers from inspiring English sources

RBIP uses simple PCR-based detection of the presence or absence of single transposon insertions by combining two primers flanking the insertion site with a single outward-priming transposon-specific primer [ 15- 17, 19, 20].

Chimeric mutants with respect to the transmembrane helix between PP1 and PP2 were generated by combining two cDNA fragments using PCR with primers at the ends of the combined sequence.

From five individuals the whole mitochondrial control region (CR) was isolated by combining two overlapping fragments: 5' (Gar-Thr1+/TDK_DGbar1); 3' (CR1+_Gbar/12SR5), the two external primers bind in the flanking tRNA-Thr gene and in the 12SrRNA gene, respectively.

This step was streamlined by combining two techniques in the same PCR reaction: (1) custom-labeling of primers by the investigator and (2) multiplexing multiple primers together in the same reaction.

In order to overcome the genetic diversity of FMD viruses (FMDV), these multiplex RT-LAMP assay pairs were established by combining four newly designed primer sets with two primer sets that had been previously published.

Comparing to monoplex PCR conditions, which generally didn't work in duplex PCR by just combining two sets of primers together, several adjustments associated with reaction chemicals were tested to optimize the protocol.

Taken together, by expanding the read depth and combining three different primer pairs for the NGS approach, we estimate that as many as 81 A. carolinensis OR sequences could be collected.

Two sets of the LAMP primers were designed for VP28 gene of WSSV and non-structural protein 1 (NS1) gene of IHHNV, in which a restriction enzyme cleavage site was inserted into two pairs of species-specific primers, to construct the mLAMP assay by combining these two sets totaling eight primers.

In this study, we designed two sets of the LAMP primers for rhoptry-associated protein-1 genes of Babesia bovis and B. bigemina, in which a restriction enzyme cleavage site was inserted into two pairs of species-specific primers to construct a multiplex LAMP (mLAMP) method by combining these two sets totaling eight primers.

The mRT-LAMP assay was designed by combining these two sets for a total of eight primers.

The REMAP markers were created by combining LTR primers with ISSR primers, as shown in Table  3.