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By combining primer sets 341 534 and Er10 Er11, all 12 of the bacteria could be separated, indicating various degrees of polymorphism within the selected primer regions.
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The TCRB sequence was generated by a single PCR with a combined primer set 34.
When combining primers from all sets PrimerROC produced an AUC of 0.91 and true negative rate of 0.69 at the dimer-free threshold.
cDNA was combined with primer sets and Power SYBR® Green PCR Master Mix (Applied Biosystems, Inc., CA).
In order to overcome the genetic diversity of FMD viruses (FMDV), these multiplex RT-LAMP assay pairs were established by combining four newly designed primer sets with two primer sets that had been previously published.
cDNA was combined with primer sets and the ABI Power SYBR Green PCR Master Mix.
Two sets of the LAMP primers were designed for VP28 gene of WSSV and non-structural protein 1 (NS1) gene of IHHNV, in which a restriction enzyme cleavage site was inserted into two pairs of species-specific primers, to construct the mLAMP assay by combining these two sets totaling eight primers.
In this study, we designed two sets of the LAMP primers for rhoptry-associated protein-1 genes of Babesia bovis and B. bigemina, in which a restriction enzyme cleavage site was inserted into two pairs of species-specific primers to construct a multiplex LAMP (mLAMP) method by combining these two sets totaling eight primers.
The mRT-LAMP assay was designed by combining these two sets for a total of eight primers.
The REMAP markers were created by combining LTR primers with ISSR primers, as shown in Table 3.
All PCRs were multiplexed by combining 3 – 4 primer pairs in each reaction.
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