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A complete PCR mixed was prepared (20 μl per reaction) by combining an equal volume of PCR primer mix (Dxterity) and 2X Dynamo probe master mix (New England Biolabs, F-450 L).
A pool was generated by combining an equal amount of extract from each cell type.
Three biological replicates per group were generated by combining an equal amount of total RNA or MT protein extracts from two randomly selected animals (Fig. 1).
Pooled IgG was obtained by combining an equal concentration of IgG with similar aPL binding from five individual samples in the VT+/PM−, VT−/PM+ and HC groups shown in Table I.
A large pooled serum sample from Egyptian donors was not available, so we prepared a small pooled sample by combining an equal volume (25 μL) of all the Egyptian noncancer comparison samples available from this study (a total of 52).
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Finally, in certain experiments virgin females were collected over 24 hours, and mating was then conducted by combining with an equal number of young (1-2 weeks of age) w[1118] strain males, for either 2 days or 4 days, as indicated.
Bulks were either performed by mixing the 3 DNAs before performing the AFLP/M-SAP assays or by combining in equal quantity 3 amplification products on a single lane of the acrylamide gel.
Agarose plugs containing bacterial cells were prepared in a 1 ml syringe by combining cells with an equal volume of 2% SeaKem Gold agarose (FMC BioProducts, Rockland, ME) melted in water.
Maximum hemolysis was achieved by combining erythrocytes with an equal volume of water.
The five new markers (wri1, wri2, wri3, wri4 and wri5) and csPSY were also assayed on the panel of 25 wheat cultivars, on the Chinese Spring nullisomic-tetrasomic lines (CS N7A-T7B, CS N7A-T7D, CS N7B-T7A, CS N7B-T7D, CS N7D-T7A and CS N7D-T7B) and on an artificial heterozygote (created by combining equal amounts of Excalibur and Kukri 50-ng/μl DNA).
A new severe plastic deformation (SPD) process of "equal channel angular swaging" (ECAS) is developed by combining the conventional equal channel angular pressing with the incremental bulk metal forming method rotary swaging.
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by combining an experimental
by combining an inverse
by combining an MFCC-based
by combining an appreciative
by combining an InterProScan
by combining an apoptosis-inducing
by combining an efficient
by combining an SVM-based
by combining an exclusive
by layering an equal
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com