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We investigated this outbreak by combining a multilocus sequence typing approach (13 ) with molecular evolutionary analyses.
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To further explore selection at age-1, we contrasted multilocus polymorphism and divergence by combining our data with a larger set of genes (Dey et al. 2012) and used the maximum likelihood HKA framework (Wright and Charlesworth 2004).
Alleles detected from polymorphic primer pairs were used to generate a chloroplast haplotype of each individual; multilocus haplotypes were obtained by combining alleles from all polymorphic loci.
While the general shape of the curve is predicted by combining data in this manner, the observed reduction in variance with additional regions indicates that most of the multilocus combinations discriminate nearly the same number of species.
By using a multilocus genotyping, a red deer group was excluded from the autochthonous population [ 43] suggesting the presence imported red deer from foreign countries.
Subtypes were identified by using a multilocus fragment-size analysis approach to target 3 microsatellite markers (ML1, ML2, and gp60 [synonymous with gp15]) as previously described (25 ).
For most purposes, that mechanism would not be altered by a multilocus model of the type described here.
The phylogenetic relationships among completely sequenced Pseudomonas were determined by a multilocus sequence analysis using a concatenated data set of gyrB and rpoD genes.
Haplotype networks were constructed for each locus and for combined multilocus sequences by statistical parsimony with the program TCS 1.21 [ 60, 61].
We have previously developed protocols to analyze GWAS data using a multilocus approach which combines bioinformatic and genetic data [ 9, 16, 17].
Analysis of combined multilocus sequences of all loci resulted in an overall interlocus genetic association (Zn s) value of 0.2711 (95% CI 0.0695 0.3918; the probability p for expected Zn s ≤0.2711 was 0.883).
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