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The crude product was purified by column chromatography using 50% ethylacetae in Hexane with 60 120 mesh silica gel to get pure 6H-benzo[c]chromene-3,8-diol product.
The crude product was purified by column chromatography using 5% ethyl acetate/petroleum ether as eluent.
The fluorescent product was purified by column chromatography using silica gel (60 to 200 mesh) and CHCl3 as eluent.
The crude products were purified by column chromatography using ethyl acetate-hexane mixture in 3 7 ratio.
The crude mixture was then purified by column chromatography using DCM/hexane as the eluents to yield 5 (0.17 g, 58%) purple solid.
The residue was purified by column chromatography using DCM/hexane as the eluents to yield the product 4 as an orange powder (0.10, 66%).
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Recombinant Hu BChE was purified to homogeneity by ammonium sulfate fractionation followed by affinity column chromatography using procainamide Sepharose and cobalt Sepharose gels.
The coding strand of the ovalbumin gene was partially purified from sheared chick DNA by affinity column chromatography using ovalbumin mRNA immobilized on phosphocellulose.
Finally, the desired products in series 6, 7 and 8 were purified by flash column chromatography using methanol/dichloromethane as the gradient eluent with overall yields of 72% 81%.
The residue was purified by flash column chromatography using hexane/ethyl acetate (4/1) as the eluent to give BT10 as a yellow solid.
For libraries 16 19, small cDNA fragments were removed by Sephadex column chromatography using a CHROMA SPIN-1000 column (Promega).
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