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The binding affinity of the imprinted polymers to the NA analogs, as studied by colorimetric methods, was found to be efficient and highly selective.
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Total flavonoid content in the extract, as measured by aluminium chloride colorimetric method was 54.72 ± 1.81 mg catechin/g extract.
TSB was measured by colorimetric methods using centrifuged venous blood samples.
Plasma total Ca, P and creatinine (Ca, P and Cr), and urinary Ca, P and Cr (uCa, uP and uCr) was measured by colorimetric methods (Cambridge: Konelab, Finland; Shenyang: 7170A Hitachi automatic analyzer, Japan).
The activities of the rh-prolidase and organophosphoric acid anhydrolases (OPAA) were assayed by colorimetric methods.
Total flavonoid and phenolic contents were determined by colorimetric methods using quercetin and gallic acid as standard.
The concentrations of NO3 −/NO2 − were determined by colorimetric methods (Lambert and Zitomer 1960; Wang et al. 2015).
The total P (TP) and available P (AP) were determined by colorimetric methods (Garg and Kaushik 2005) and resin extraction with modification (Hedley and Stewart 1982), respectively.
Serum triglycerides (Infinity triglycerides kit, Thermo), free fatty acids (half micro test kit, Roche), β-hydroxybutyrate (Stanbio Laboratory), and lactate (BioVision) were determined by colorimetric methods according to the manufacturer's instructions.
Total iron, Fe3+ and Fe2+ were determined by colorimetric methods [ 53].
The total phenol and flavonoids contents of the HSME were quantified by colorimetric methods.
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