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Free HRP and the immobilized HRP activity was assayed by colorimetric method using 4-AAP as previously described [2].
Serum was analyzed for total antioxidant capacity by colorimetric method using Diagnostic and Research Reagents of Biodiagnostic, Egypt (CAT. No.; TA 25 13).
PCP concentrations in solution were measured by colorimetric method using UV Vis spectrophotometer (DR 5000) in 500 nm wavelength (Asgari et al. 2014).
Cholesterol and triglycerides were determined in spectrophotometer by colorimetric method using a commercial KIT (Labtest Diagnóstica S/A, Lagoa Santa, MG, Brazil).
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Total flavonoid and phenolic contents were determined by colorimetric methods using quercetin and gallic acid as standard.
TSB was measured by colorimetric methods using centrifuged venous blood samples.
Lipid peroxides (LPO) were detected by colorimetric methods, using a kit (Jiancheng, Nanjing, China) according to the manufacturer's instructions.
Serum calcium, phosphorus, alkaline phoshatase and other biochemical parameters were measured by colorimetric methods using a Roche autoanalyzer or other standard laboratory techniques.
The concentrations of TC, calcium, alkaline phosphatase, phosphorus, triglyceride, and HDL cholesterol were measured by colorimetric methods using a Toshiba 200FR automated analyzer (Toshiba Medical Systems, Tokyo, Japan).
Nutrients such as the nitrite (NO2) was measured by a colorimetric method using sulfanilamide, nitrate (NO3) by the cadmium reduction method, inorganic phosphorus (IP) and total phosphorus (TP) by the ascorbic acid method and reactive silicate by the molybdate method using a PC-based double-beam spectrophotometer Systronics-22022).
The total carbohydrate content was analyzed by a colorimetric method using anthrone reagent (Updegraff [1969]).
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