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The total phenolic content was evaluated by colorimetric analyses using Folin Ciocalteu's phenol reagent (Singleton and Rossi 1965).
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Therefore, caspase-3 and -8 activities were determined by colorimetric analyses.
Dephosphorylation of these peptides by SCP3, was analysed using a colorimetric phosphate release assay.
Nitrate concentration in soil was analysed on weekly collected samples by colorimetric technique using Reflectoquant® strips read by a reflectometer RQflex® 10 (Merck, Darmstadt, Germany).
Blood glucose, serum cholesterol and TAGs (triacylglycerols) were measured by colorimetric kit assays (Leadman) and analysed using a fully automatic biochemistry analyser (Hitachi).
Glycogen content was measured by colorimetric reaction using 25 μL of prepared sample.
Cell proliferation was assessed by colorimetric assay using 3- 4,5-Dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide (MTT) and BrdU (colorimetric) kit (Roche Diagnostics GmbH, Germany).
DNA-binding activity of CREB was also determined by colorimetric assay using the TransAM ELISA kit.
All samples were analyzed by colorimetric assays using commercially-purchased kits (Creatinine and Sensiprot Kits, Labtest, Minas Gerais, Brazil).
Proteins were visualized by colorimetric reaction using opti-4CN, (Biorad) and analyzed using QuantityOne Software (Biorad).
After 72 hours incubation at 37°C, cell viability was determined by colorimetric assay using CCK-8.
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