Sentence examples for by collection of buffer from inspiring English sources
For glucose-stimulated insulin secretion, cells were incubated in HBSS for an additional 2 hours in the presence of 3 mM, 15 mM glucose or 15 mM glucose+30 mMKCl as indicated followed by collection of buffer for insulin radioimmunoassay (Coat-A-Count kit, DPC).
A buffer family is a collection of circular buffers which store time series data for a single variable.
De novo infections with M1000 were prepared by centrifugal collection of M1000-infected and mock-infected cells (300 g for 5 minutes), followed by washing in Dulbecco's phosphate buffered saline (dPBS; Invitrogen) and centrifugation as before.
Cells were washed two times with PBS and resuspended in 1 ml of collection buffer.
"Buffer" is the Travelers' term for civilians, and the Malloys are at first bewildered by and slightly contemptuous of buffer luxuries like the garbage disposal and flat-screen TV.
Bound protein was eluted in glycerol elution buffer (20% glycerol, 50 mM TrisHCl pH 8.0, 500 mM NaCl, 250 mM imidazole, 2 mM 2-mercaptoethanol, 5 µg/ml aprotinin and 5 µg/ml leupeptin) using three separate 0.1 ml additions of elution buffer and collection of eluate by centrifugation.
Like a collection of by-elections perhaps?
Before collection of CD data, proteins were buffer-exchanged into CD-appropriate buffer (50 mM Tris/H2SO4, pH 7.5, 200 mM K2SO4 and 1 mM DTT).
Sample injection was performed manually by on off switching of the syringe pump delivering buffer 2. In the diffusion-based separation and the CE separation mode, three-layer flow composed of sample solution, collection buffer (buffer 1), and buffer 2 occurs in the 300 μm square-PDMS channel along the buffer 2 flow channel, as illustrated in Fig. 1.
The passivation surface decreased the total interfacial electron localized state and electron transfer barrier, which could re-modulate the interfacial property of buffer layer and facilitate electron collection.
