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In addition, we confirmed recently published findings that EGFP-LC3 is sequestered in the cytoplasm by coexpression of a mutant form of Atg4B, a protease that cleaves the LC3 precursor to produce LC3-I and that catalyzes the delipidation of LC3 [25].
As expected, such phenotype could be suppressed by coexpression of a wnd RNAi (data not shown).
Previous reports showed that a repressed amplicon could be activated by coexpression of a strong silencing suppressor [ 16].
Enhancement of the immune response was also obtained by coexpression of a Salmonella enterica gene encoding flagellin, a protein-stimulating TLR5 receptors, and a gene encoding the Gag HIV-1 protein in an L. acidophilus strain (Kajikawa et al. 2012).
Cells transformed by coexpression of a constitutively activated FGFR2 mutant plus c-Myc appeared to be become highly addicted to FGFR-dependent prosurvival activities, as small molecule inhibition of FGFR signaling resulted in robust p53-dependent apoptosis.
N-Terminal acetylation of WT aS was achieved by coexpression of a plasmid carrying the components of the NatB complex with a plasmid containing the wild-type aS gene, following the protocol described by Johnson et al. We observed that the restrictive conditions of M9 media have a strong effect on the acetylation reaction.
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Here, we demonstrated that ABF-2 could be expressed as an inclusion body in large quantities by coexpression of an aggregation-prone protein.
By contrast, the phenotype is enhanced by coexpression of an oncogenic form of Ras.
The rough eye phenotype of these flies was enhanced by coexpression of an RNAi (IR) construct against mbl (UAS-IR-mbl) generated in our laboratory.
From our initial screening by coexpression of an iPS factor with either p300 or CBP in cells, we observed various acetylation patterns among these iPS factors.
We have described a recombinant expression system that provides facile access to both major isoforms of the highly amyloidogenic Aβ peptide by coexpression of an engineered aggregation-inhibiting binding protein.
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