Sentence examples for by codon optimization and from inspiring English sources

Figure 2C also shows that translational enhancement by codon optimization and by use of ARCA cap is additive.

Compared to previous generations of luciferase, the new version (luc2) delivers more than a four-fold increase in light emission which was achieved by codon optimization and removal of potential transcription factor binding sites[16].

Full-length human CL3 was synthesized by codon optimization and carried Flag and HA tags at identical positions as in CL1.

In this work, the expression of the hydantoinase from Arthrobacter crystallopoietes DSM 20117 was optimized by codon-optimization and furthermore, a screening for the indirect induction of chaperones as well as for coexpression of different chaperones was carried out to improve soluble hydantoinase expression.

Previous studies have found that the rare codons have the greatest degree of conformity to lowly expressed genes in the E. coli cell, and the expression level may significantly be improved by codon optimization (Sharp and Li 1987; Peng et al. 2004; Xu et al. 2006).

Since human papillomavirus (HPV) E6 and E7 are promising tumor antigens, we engineered E6 and E7 antigens to generate an optimal HPV DNA vaccine by codon optimization (Co), fusion of E6 and E7, addition of a tissue plasminogen activator (tpa) signal sequence, addition of CD40 ligand (CD40L) or Fms-like tyrosine kinase-3 ligand (Flt3L).

By codon optimization, the expression of xylanase gene from Thermotoga maritime and Aspergillus sulphureus was improved 2.8- and 5-fold, respectively [ 18, 22].

This result suggested that cryptic 3′-end-processing signals are recognized by A. oryzae within the coding region of the heterologous gene and that these signals are eliminated by codon optimization.

Our results demonstrate that the vast majority of enhancement of protein expression achieved by codon optimization of DNA vectors is unrelated to codon usage per se, and actually mediated by pre-translational effects, such as improved mRNA stability and transport.

In C57BL/6 primary mouse T cells, the expression level of WT and cysteineized TCR formats could be considerably increased by codon optimization (Fig. 5A).

Increasing evidence suggests that the enhancement of Gag expression by codon optimization in DNA-transfected cells is largely attributable to changes in nuclear RNA stability and export, rather than to enhanced translational efficiency [10], [12]-[14].