A 200-fold increase in transposition in the mouse germline has also been achieved by codon optimisation of the human L1 coding region [270].
We have shown that, by using a stepwise strategy to address specific problems relating to codon optimisation, periplasmic protein translocation and tight regulation of expression, efficient bacterial expression of DI of human β2GPI is possible.
Various isoforms of invertases from prokaryotes, fungi, and higher plants has been expressed in Escherichia coli, and codon optimisation is a widely-adopted strategy for improvement of heterologous enzyme expression.
We demonstrated the positive effects on protein yield of plant codon optimisation, specific intracellular targeting, and protein composition.
Methods for increasing protein yield include codon optimisation of the genes, and fusion of signal sequences to target recombinant proteins to subcellular compartments [ 18, 19].
Other parameters such as GC-content bias, stability of the RNA secondary structure and length of direct and inverted repeats were also assessed following codon optimisation.
Codon optimisation resulted in a 30-fold, 2.5-fold and 4-fold improvement in the signal for FFluc, Gluc and Lux respectively.
Genes encoding VH and VL of hHMFG1, HT186-B7, -D11 and -G2 were orderd as synthetic genes with codon optimisation for rat (Genscript, Piscataway, NJ, USA).
We demonstrate substantial synergy when such gene amplification is combined with extremely high codon optimisation strategies.
The yields of H5 and H5tr varied with experiment and by transgenic plant line, but in general were higher than those obtained by others: this may be due to the vector system used, as well as to the human codon optimisation of the genes.
