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First, sequence redundancy was removed by clustering the duplicates using CDHIT-EST at 95% sequence similarity [ 34].
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The PCA results were confirmed by clustering.
Coexpression should reflect common gene function and local coexpression may be partially explained by clustering of duplicated genes.
HNF4α-induced protein expression by the duplicate 4xH4RE Hnf1a promoter was comparable to a viral promoter.
Furthermore, the risk of clustering non-orthologous gene duplicates into a given UniRef50 cluster is reduced in nematode-trapping due to the rapid divergence of gene duplicates generated by repeat induced point (RIP) mutations [ 19, 20].
Although output transcripts are clustered and duplicated transcripts are removed, many duplicates are difficult to detect and errors can accumulate in the cluster-remove step.
The clusters include duplicates as well as singleton tRNAs with no clear pattern associated with the distribution of singletons, duplicates and triplicates.
We estimated the number of gene families and the members of each family by a single linkage clustering of duplicated genes for each species.
The solution to the inter-cluster duplicate coverage problem necessitates the neighbor clusters to exchange their CSP values, member list, target IDs covered by the members, and their TCP values.
Since this dataset was already filtered by the original authors, we skipped the quality control, duplicates clustering and FILTER-HUMAN steps.
As expected, the duplicated cotton Hsfs clustered on the same group.
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