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Open image in new window Fig. 17 Identification of THU and SHU by cluster analyzing method.
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The density of repetitive sequences (calculated by dividing the total length of repetitive sequences by the length of the clusters analyzed) in the caecilian Hox clusters was 3.3 %, among the lowest densities of all the species studied (Fig. 2).
To account for multiple hypothesis testing, we divided our original significance threshold (P < 0.01) by the number of putative clusters analyzed (103), establishing a corrected significance threshold of P < 9.7 × 10−5.
Herein, we performed an evolutionary study of the ALR cluster by analyzing both inter- and intraspecies diversity.
In the following, we will derive the optimal number of clusters by analyzing the energy model introduced in Section 3.2.
It was then determined whether there was a statistical difference in the ratings between these clusters by analyzing the ratings with an ANOVA similar to the one described above.
Recently, a method for predicting accurate margins of SMB gene clusters by analyzing the co-expression of neighboring genes has been reported, with the condition that the gene indispensable for the SMB gene cluster is identified using the known sequence of the core gene.
In order to identify patterns of gene expression commonly regulated during both daytime and night-time heat stress, the 5653 detected transcripts were allocated into 8 clusters by hierarchical clustering before analyzing the relative enrichment of functional categories (Additional file 5).
Cluster indicates the cluster number analyzed by k-means clustering (k = 30).
(5) Z-score values for each same-same codon doublet in each gene, along with data describing whether a gene was smaller or larger than average, were imported into the program CLUSTER and analyzed by hierarchical clustering (17).
In order to test to test the accuracy of our clustering approach and cut-offs, we simulate a clustering fidelity by analyzing how frequently fragments from the same genome are clustered together when a pool of phylogenetically unrelated 15 kb sequences are compared.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com