Sentence examples for by cluster amplification using from inspiring English sources
Both sRNA sequencing libraries were normalized to 2 nM with Tris HCl, denatured using NaOH, diluted to 7 pM using pre-chilled Illumina TruSeq Hybridization Buffer and hybridized onto an Illumina Paired-End Flow cell followed by cluster amplification using an Illumina cluster station with a TruSeq Cluster Generation Kit V5.0 as per the manufacturer's instructions.
The amplified libraries were purified by AMPure (Beckman Coulter) purification and hybridized to an Illumina paired end flow cell for cluster amplification using the cBot (Illumina) at a concentration of 8 pmol per lane.
The amplified libraries were purified by AMPure (Beckman Coulter, Brea, CA) purification and hybridized to an Illumina paired-end flow cell for cluster amplification using the cBot (Illumina) at a concentration of 8 pmol per lane.
Using an Illumina Cluster Station, the diluted libraries were hybridized to an 8-lane flow cell, followed by cluster generation by isothermal amplification using a DGE-Small RNA Cluster Generation Kit v1.0 (Illumina, San Diego, CA).
A total of 130 clusters was obtained, and a total of 74 of those was tested to determine their host origin by PCR amplification using sunflower and downy mildew genomic DNA.
The vectors were prepared by PCR amplification using pCC1BAC (Epicentre) or p15A vectors as templates, followed by DpnI (NEB) treatment38.
Random whole genome amplification (WGA) by isothermal amplification using the Phi29 DNA polymerase has established itself as an excellent alternative to random-PCR based amplification.
GF polyp macerates were further analysed by 16S rDNA amplification, using universal 16S rRNA primers Eub-27F and Eub-1492R66.
Labeling and further amplification were then performed by classic PCR amplification using biotinylated primers specific for the linkers.
Additionally, accurate amplification is achieved by reduction of nonspecific amplification using a unique asymmetrical primer set.
In vitro amplification using the polymerase chain reaction followed by oligonucleotide hybridization can be used to detect murine CMV DNA.
