Sentence examples for by cloning the coding from inspiring English sources
A prokaryotic RBM24 expression plasmid pET33b-EK-RBM24 pET33b-EK-RBM24 pET33b-EK-RBM24ding sequence of RBM24 into pET33b.
The plasmid pGST-ACP-INC was obtained by cloning the coding sequence for the Ssp GyrB S11 IN (followed by the chitin binding domain) downstream of the V. harveyi ACP in plasmid pGEX-F50W [40].
The efficacy of coding seed match sites was tested for two mRNAs lacking seed matches in their 3'-UTR by cloning the coding sequences in-frame with a luciferase reporter; mutation of these seed match sites led to a ∼25% increase in the expression of luciferase.
His-tagged EaeA was produced by cloning the coding region of eaeA into pET16b (Novagen, Gibbstown, NJ).
The CHD5 expression plasmid was constructed by cloning the coding region of CHD5 into the pcDNA3.0 vector (Invitrogen).
FLAG-tagged Sp1 was expressed by cloning the coding sequence of Sp1 cDNA ([NM_013672] for mouse and [NM_138473] for human) into the pCMV-3Tag-1 vector (Agilent Technologies).
pcDNA4/TO/myc-HisB-APOBEC3G (A3G) was constructed by a cloning the coding sequence of A3G into pcDNA4/TO/myc-HisB such that A3G is tagged with the myc-epitope at the C-terminus.
The YFP-B-Myb expression vector was obtained by cloning the complete coding region for mouse B-Myb downstream of the coding region for the YFP in plasmid pYFP-C1.
The ss-GFP cDNA was generated by cloning the GFP coding sequence 3' and in frame with the ss coding sequence.
Overproduction of each candidate Chlamydomonas cell surface specific sdAb antibody was achieved by cloning the VHH coding region into the pET32b overexpression vector downstream of coding sequences for thioredoxin A and 6 × His (for recombinant protein purification) and upstream of the coding region of an E-tag epitope.
A stably transfected cell line expressing human S1PR3 was generated by cloning the sequence coding for human S1PR3 (NCBI accession number NM_003226) together with the sequence coding for the human G-protein Gα15 (NCBI accession number M63904) into a plasmid vector suitable for eukaryotic expression (pVITRO2; Invivogen).
