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We have initiated this study by cloning a functional form of Chikungunya virus E2 envelope protein.
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This approach could also be applied to screen for target sequences for a known protease by cloning random sequences in between the two functional domains (72).
Therefore our goal here was to carry out a genome-wide and functional analysis by cloning and expressing every viral gene encoded by the HIV genome in fission yeast.
Functional promoter analysis is normally performed by cloning part of the 5' upstream region of the gene into an appropriate reporter vector, such as used for luciferase assays.
Corporate contributors make a mockery of campaign finance rules by cloning themselves to circumvent contribution limits.
Two human NATs, NAT1 and NAT2, have recently been characterized by protein purification, cloning, and functional expression of the respective genes; both were localized to chromosome 8.
The resulting 114 independent mutant clones were characterized by a functional complementation assay, and were shown to be defective in any of 20 genes among all 22 known genes essential for this well-characterized pathway.
In 2009, the B73 maize genome sequencing made a great step forward, using clone by clone strategy; however, functional annotation and gene classification of the maize genome are still limited.
Translation of the second cistron, reported by enhanced yellow fluorescent protein (eYFP), is inefficient unless the cloned UTR contains a functional IRES.
The sequence information is a useful resource for identification, cloning and functional genomic studies in future.
The corresponding PEX genes were cloned by functional complementation upon transformation of the selected mutant with a genomic library.
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CEO of Professional Science Editing for Scientists @ prosciediting.com