Sentence examples for by cloning a fragment from inspiring English sources

The pcDNA6.2-GW/EmGFP-miRNA 17-5p contructcontruct was obtained by cloning a fragment PCR amplified from human genomic DNA into pcDNA6.2-GW/EmGFP-miR vector.

The mitochondrial target sequence was added by cloning a fragment in front of GFP or GST using NheI (in pUAST2) and MssI (in GFP and GST).

The lefty2 RNA in situ probe was made by cloning a fragment of the cDNA.

pcDNA3.1-Isl1 was generated by cloning a fragment encoding C-terminal 216 amino acids of Isl1 into the pcDNA3.1/Hygro vector.

Plasmid pRS305 - P GAL1 10 -POL30-3myc was constructed by cloning a fragment containing POL30-3myc (amplified by PCR from genomic DNA prepared from TKY212) into pRS305- P GAL1 10, using In-Fusion HD cloning (Takara Clontech).

For localisation studies, we constructed the expression plasmid pcDNA-TSPY-FLAG by cloning a fragment containing the full-length ORF minus the termination codon in the HindIII– BamHI sites of pcDNA-FLAG vector.

Vector pDXA-CTD was generated by cloning a PCR fragment encoding CbfA724 998 (primers CMBF-37.3 and CMBF40.1, see Table S4) into the KpnI and XhoI sites of pDXA-3H [26].

Similarly, plasmid pDmsALT18 was constructed previously by cloning a PCR fragment encoding the signal peptide of E. coli dmsA (excluding the signal peptide cleavage site) into the PstI and KpnI sites of pUT18 [43].

The p-value for being GC rich was obtained by selecting randomly, 10000 times, groups of n genes and calculating the fraction of such groups that had GC percentage higher than P. SM22-luciferase reporter constructs were generated by cloning a genomic fragment of the SM22 gene spanning from 166 to −57 bp relative to the TSS into a pGL3 luciferase reporter vector (Promega).

Plasmid pFL36.1 was obtained by cloning a RAD9-3HA fragment into XhoI- NotI-digested pRS306.

Both partial genes were obtained by cloning a PCR fragment of approximately 950 bp.