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Such a low percentage of cells stably expressing a transgene can be bypassed by clonal selection when working with immortal cell lines.
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Reflecting the genesis of tumors as an evolutionary process driven by clonal selection.
Self-reactivity was once seen as a potential characteristic of T cells that was eliminated by clonal selection to protect the host from autoimmune pathology.
An algorithm for resource allocation in cloud computing environments inspired by clonal selection algorithm is proposed.
A limitation of the present work is represented by the low number of cells obtained by clonal selection, resulting in restrictions to the possible analysis.
Despite these encouraging data, the low number of cells that can be isolated by clonal selection make this approach hardly applicable to cell-based therapy, until the identification of growth factors able to enhance the cell yield from isolated clones.
On the other hand, the number of cells obtained by clonal selection was extremely low and most of the clones were unable to reach a high number of passages in cultures.
A stable cell line overexpressing Grx1 was generated by electroporating SH-SY5Y with 20 µg of linearized construct overexpressing Grx1, followed by clonal selection.
Alternatively, they may be generated by clonal selection.
The main conclusion from the genomic study is that breast tumours undergoing neoadjuvant endocrine therapy show clonal variation that could be explained by clonal selection.
Because multiple rounds of mutation are followed by clonal selection during tumor evolution, selection against cells with lethal combinations of mutations will produce mutual exclusivity.
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