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As in the larval body size experiments described above, fat body cell size was unaffected by clonal expression of the negative control constructs UAS-lacZ-RNAi (Figure 2D F and 3A) or UAS-lacZ (Figure 2J L and 3B).

Furthermore, clonal expression of HD imposed by either blastomere transplantation experiments or gene transfer assays unequivocally highlights that Hbox12 action in presumptive dorsal ectoderm cells is necessary to harmoniously pattern the embryo along its DV axis.

By contrast, and in accordance with the previous result, either the ectopic or clonal expression of the hbox12 mRNA specifically prevents p38-GFP nuclear translocation.

The resulting clonal expression of GAL4 then activates transcription of stably integrated RNAi and GFP expression constructs driven by similar GAL4-responsive promoters.

In addition, clonal expression of Gal4 can be achieved through the use of the recently developed MAZe technology.

This destabilization would be expected to result in non-clonal expression of various mutations.

During the initial phase of mitotic clonal expression the phosphorylation of Rb was induced; however, this phosphorylation step was not modulated by BSO (Figure 5B).

Mitotic clonal growth is accomplished by increased expression of CCAAT/enhancer binding protein beta (C/EBPβ) which further stimulates the expression of two transcription factors, C/EBPα and PPARγ involved in adipogenesis and lipogenesis [ 36].

Further improvement in the fusion efficiency can be expected by clonal selection of donor cells with a high level of MV-F/H expression.

In addition, NK cells derived by clonal expansion of a single seed cell were relatively homogeneous in their expression of HLA-DR.

An Sn-expressing cell line, TSn, was generated by clonal expansion of a single transductant.