Gcn5 plays several different roles in gene transcription throughout the genome but their characterisation by classical mutation approaches is hampered by the high degree of apparent functional redundancy between HAT proteins.
Elevated basal, ligand-independent, Wnt signaling in some canine breast cancer cells is not caused by classical mutations in APC, β-Catenin or GSK3β but, at least partially, by enhanced LEF1 expression.
The disease is not caused by classical mutations in a protein-coding gene, but it correlates with reduction in the copy number of the 3.3 kb macrosatellite D4Z4 repeat mapping in the subtelomeric region of human chromosome 4 long arm (4q35).
In the article by Kevin McCluskey, Aric E. Wiest, Igor V. Grigoriev, Anna Lipzen, Joel Martin, Wendy Schackwitz, and Scott E. Baker (G3: Genes | Genomes | Genetics 1: 303−316) entitled "Rediscovery by Whole Genome Sequencing: Classical Mutations and Genome Polymorphisms in Neurospora crassa," a gene is incorrectly labeled.
Interestingly, a recent survey on classical mutations and genome polymorphisms in N. crassa [ 36] revealed significant genomic variation in classical mutant strains in this fungus.
A corresponding classical mutation can facilitate QTL isolation and the understanding of QTL gene function.
Moreover, compared to classical mutation screening of GRN by sequencing analyses, a GRN ELISA has the important advantage of identifying individuals carrying all types of mutations including GRN deletions.
On the other hand, it is much faster to evolve a new crRNA, which can be produced by a Lamarckian mechanism, than a new RM system, which requires classical Darwinian evolution by random mutation and selection.
The term epigenetics denotes heritable phenotypic alterations caused by postreplicative modifications of chromatin, rather than classical mutation-based genetic changes.
In this article, we report the whole genome sequencing of seventeen classical mutant strains of N. crassa, and the putative identification of classical mutations in sixteen of them.
