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Gender was determined by checking the gonads.
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Start by checking the toenails.
At this step, we also verified that the lin-12 array was lost in the gonad by checking for loss of mir-84p::YFP expression.
Check the sell-by date.
Check the eggs by opening the carton.
Check the facilities offered by the university.
Briefly, sperm and oocytes were collected in seawater by stripping the gonads.
To develop a CV that would hopefully grab potential employers by the gonads and shout 'HIRE ME' like it should do, I began to adapt my CV to suit each application individually.
Sex and maturation status were visually determined by inspection of the gonads.
Cytokines also affect gonadal sex steroid production by acting directly on the gonads [ 45].
Sex for each fish was determined by macroscopic examination of the gonads.
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