Sentence examples for by checking the expression from inspiring English sources
The confirmation of transfection was examined by checking the expression of EDA in control (non vector), vector control and EDA vector transfected T47D cells by reverse transcription-PCR and western blotting (Fig. 6a,b).
We have also excluded that any aberrant event could account for the presence of nestin+/GFAP+ cells during differentiation at 1% oxygen, by checking the expression of early markers specific for neuronal and astroglial lineages.
The polarization was verified by checking the expression of polarization marker genes for Th1 and Th2 subsets.
siRNA validation was carried out at 24 h by checking the expression of TREK-1 via western blot experiments.
We also performed RT-qPCR analysis to validate our Northern results by checking the expression pattern of miR167 and miR482 in N. benthamiana tissues.
Furthermore, by checking the expression differences after stress treatments in callus, we also observed that part of the LSGs expressed responsive to a variety of abiotic stresses.
Strand-specific quality evaluation was done by: (1) checking the expression correlation of protein-coding genes between two strands within each sample; (2) examining the sense/antisense ratio of the exon-spanning junction reads that had built-in directionalities (Additional file 5: Figure S9, Additional file 15: Table S12 and Additional file 3: Supplementary Methods).
Its expression was further substantiated by checking the level of protein expression of TNFSF10 by western blotting in the transfected PC3 cells.
The later are subsequently combined to compose complete gene expression events by checking the presence of both a 'Gene' and a 'Cell' relationship linked to the same trigger.
By checking the association between VEGFA and TGFBR2 expression using the TCGA BRCA dataset, we detected an opposite effect to that seen with CXCL1, MCP-1 and IL-6.
Conservation of gene expression was compared by checking the 3,892 1∶1∶1 orthologous genes in mouse, chicken and frog (Table S5).
