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In Caenorhabditis elegans, sex-regulated genes have been identified by characterizing gene expression differences between males and hermaphrodites using DNA microarrays [24], while in Drosophila melanogaster, microarray analysis has been performed to obtain the gene expression profile of the testis [25].
The multi-step process of carcinogenesis can be more fully understood by characterizing gene expression changes induced in cells by carcinogens.
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The first dataset that we use has been recently published by [ 12], and characterizes gene expression signatures in acute lymphocytic leukemia (ALL) cells associated with known genotypic abnormalities in adult patients.
We then illustrated the utility and technical reliability of the NeuroStem Chip by characterizing the gene expression profile of commonly utilized hESC line SA02 (Sahlgrenska 2; [ 27]), including (i) undifferentiated cells and (ii) cells committed towards neuronal/dopaminergic differentiation pathway.
Furthermore, reaction expression profile can be mathematically characterized by gene expression data based on logical relations of gene reaction described in the model as follows: Thus, the ratios of logarithmic reaction expression data for strain NZN111 to that of wild-type E. coli were calculated to better indicate the differential expression reactions.
Next we tried to identify biological features, characterized by gene expression, that might be correlated with the 2nd PC.
Individual differentiated biological states can be characterized by gene expression profiling.
Cluster 12 (N = 214) is characterized by gene expression levels specifically down-regulated (from D27) in androgen-treated females.
To confirm Notch pathway activation, a profile of genes upregulated following release from gamma-secretase inhibition was characterized by gene expression arrays.
Lymphoma cases represented on the left side of the heatmap are characterized by gene expression comparable to unstimulated cells in vitro (BL2).
Overall, this data set, augmented by meta-analysis of other recent high-throughput proteomic studies of other human cell lines, provides a new insight into cell identity as characterized by gene expression at the protein level.
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