The length of the linkers can be easily adjusted by changing the linker-unit repeat-number, such as (EA3K), to achieve an optimal distance between functional units.
We have also constructed a series of FtsZ-mEos2 fusion proteins by either changing the linker sequences between FtsZ and mEos2 or by placing mEos2 at the N-terminus or internal positions of FtsZ.
Chimeric receptors (chimeras of anti-fluorescein (FL) scFv and an engineered c-Mpl receptor possessing only signaling mediator STAT3-binding motifs) were designed by changing the peptide linker length between the binding motifs of JAK and STAT3 using flexible linkers (G4S n (n = 0, 3, 6, 9).
To compare the varying dependencies for run length and velocity on internal strain, we took advantage of DNA-kinesin, whose distance between the heads and also the spring character between heads can be independently changed by changing the DNA length and linker length, respectively.
In particular, tuning of electron density by incorporation of electron-withdrawing groups, altering the nature of the PET "switch", and changing the linker between the chelate and fluorophore can tune the PET efficiency, thus influencing the relative brightness of the sensor in the unbound and bound state.
Also, changing the linker to −CH2CO (14c) or −COCH2 (15c) resulted in loss of activity.
Thus, changing the linker position at DB2275, to give DB2277, results in a significant strong and selective exothermic enthalpy that accounts for highly favorable Δ Gb° for DB2277.
Current studies are aiming to synthesize JAHA analogues by changing the cap (metal/coligands), linker, and binding groups for SAR studies and employing these analogues as structural probes.
