Sentence examples for by changing the differentiation from inspiring English sources

After induction towards one of these lineages, they retain the ability to be committed to the other one by changing the differentiation medium and cocktail for a limited time.

Differentiation was initiated by changing the media to differentiation media and following feeding schedule seen in Supplementary Tables 2 and 3. S6 basal media used in stage 6 was made by supplementing CMRL 1,066 Supplemented (Mediatech; 99-603-CV) with 10% fetal bovine serum (HyClone; 16,777) and 1% Penicillin/Streptomycin.

When C2C12 cultures reached 70 90 % confluency they were differentiated by changing the media to differentiation medium consisting of DMEM supplemented with 2 % horse serum, 200 U/ml penicillin and 200 µg/ml streptomycin.

The same amount of TNF-α was added every 24 hours without changing the differentiation medium.

This reduction was explained by the suppression of immune reactions, especially by changing the function or differentiation of DC and regulatory T-cells [ 29].

Polarization was changed by changing the orientation of the laser.

C2 mouse myotube cultures were prepared by growing myoblasts (purchased from ATCC, Manassas, VA, USA) on glass coverslips or in culture dishes in DMEM containing 20% fetal bovine serum (growth medium) until confluence and then inducing differentiation by changing the medium to DMEM containing 2% horse serum (differentiation medium); fresh differentiation medium was added each day for 4 5 d [53].

A general approach has been to try to change the phenotype of various cell types in vitro by changing the environment and adding growth and differentiation factors.

Differentiation was initiated 3 days after re-plating on day of differentiation (DoD) 0 by changing the medium to knockout serum replacement medium (KSR) (Knockout DMEM with 15%% knockout serum replacement, 2 mM Glutamax, 0.1 mM MEM non-essential amino acids and 50 μM beta-mercaptoethanol) supplemented with 35 ng/ml noggin, 600 nM dorsomorphin and 10 μM SB-431642.

Differentiation was induced by changing the medium to DMEM containing 2% horse serum (HS) and 1% P/S/A when the cells attained 90% confluence [ 9, 22].

At passage 2 3, Schwann cell differentiation was induced by changing the medium to standard culture medium without FBS containing 1 mM β-mercaptoethanol (BME) for 24 h.