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We succeeded in morphology-selective preparation of single-wall carbon nanohorn (SWCNH) aggregates by changing the buffer gas (760 Torr) in CO2 laser ablation of graphite: He for seed type, N2, Ar and Ne for dahlia type, and Kr for petal dahlia type.
The 70S ribosomes from the parental strain (A19) were separated into 30S and 50S subunits by changing the buffer into the dissociation buffer (same as gradient buffer, except 2 mmol/L MgCl2).
The parameters of the mixtures are updated by changing the buffer of the background values in FIFO order by selective update, and the covariance (in this case, a diagonal matrix) is estimated in the time domain by analyzing the set of differences between two consecutive values.
In this section, we carried out an experiment in order to analyze the different performance obtained by changing the buffer size Q m a x from (0.5×104) bit to (5×104) bit with a step-size of (0.5×104) bit.
Further separation was done with a Proswift SCX-1S Analytical column (4,6×50 mm, 066765, Dionex, Sunnyvale, CA) and by changing the buffer to 10 mM phosphate buffer (pH 7.01) in the third column the creatinine molecule is rendered neutral, reducing the retention in the column and focusing it, thus producing a sharp and well-defined peak with absorbance at UV 234 nm.
The hArc populations of different sizes were interconverted by changing the buffer conditions.
Similar(51)
After elution, the proteins were dialyzed (3.5 kDa cutoff, SpectrumLab, CA) in 20 mM sodium phosphate buffer, pH 7.4, and 0.5 M NaCl at 4°C overnight and the following day by changing the dialysis buffer once.
Three baseline samples were then collected followed by changing the perfusion buffer to aCSF containing 60 mM KCl (NaCl concentration was reduced accordingly to maintain osmolarity) and four samples were obtained.
The precipitated protein was dissolved in 200 ml of distilled water and dialysed extensively against 50 mM TBS (pH 7.2) by changing the dialysis buffer three times at 4°C overnight.
Large pH deviations sensed by the PL intensity when alternately changing the buffer from pH 10.0 to 5.5 (8 cycles) (e).
OG was removed by extensive dialysis using 14 kDa membranes (Sigma-Aldrich) in 50 mM MES, 50 mM Tris, 1 mM EDTA (pH 7.3) buffer, changing the buffer solution every 4 8 hours for two days.
More suggestions(16)
by changing the separation
by modifying the buffer
by changing the barrier
by changing the interface
by testing the buffer
by changing the anodization
by sensing the buffer
by changing the way
by changing the volume
by changing the accessibility
by tuning the buffer
by limiting the buffer
by changing the distribution
by replacing the buffer
by changing the oil
by avoiding the buffer
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com