To completely inactivate the enzyme so that it could form a stable complex with the diubiquitin substrate and the UIM-SH3 construct, we had to introduce an additional mutation by changing an active-site zinc-coordinating Asp to Ala.
TM can form a complex with thrombin and essentially converts thrombin from a procoagulant to an anticoagulant by changing its substrate specificity.
In light of that, researchers have been developing different approaches to artificially increase the chemical diversity of NRPs by changing the substrate specificity of A domains.
Variety of morphologies was achieved by changing the substrate pre-deposition treatment and the deposition conditions.
The first important topic is that the FZO thin films were deposited by reactive RF magnetron sputtering on glass substrate by changing the substrate temperature.
Graphane's derivatives were not only just about functionalization of the surface atoms, but also by changing the substrate atoms to achieve its function.
In addition, this novel sensing system is potentially universal as the recognition element can be conveniently designed for other target analytes by changing the substrate of DNA MTase.
Further, this antenna is made to resonate at dual frequencies of 2.48 THz and 3.35 THz, by changing the substrate height, which is reported for the first time.
Therefore, we can fabricate many derivatives of graphane by changing the substrate atoms (like C, Si, Ge, P) and the surface atoms (like H, –OH, -NH2, He, Li, Fe, Mn, and all the VII A element).
The amount of Ni in the composite powders was varied from 36 51 wt.% by changing the substrate powder loading in the electroless bath.
