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In initial experiments, we explored whether thymocyte differentiation is accompanied by changes in expression of CD71 and CD98. Figure 2A shows that DN3 thymocytes express low levels of CD98 and CD71, whereas surface expression of these nutrient transporters is high in DN4s.
A prevailing view in evolutionary developmental biology (Evo-Devo) is that morphological traits evolved mostly by changes in expression patterns of functionally conserved genes rather than through the emergence of new genes (Doebley and Lukens 1998; Carroll 2008).
This raises the possibility that epigenetic changes might regulate neutrophil function by changes in expression of microRNAs.
De-regulation of dorso-ventral patterning in β-cateninstab embryos is paralleled by changes in expression of Shh and Gli1 in the midbrain.
One hypothesis is that the therapeutic effect of valproate in bipolar disorder may be mediated by changes in expression of neuroprotective genes.
Since we found no changes in tumor progression in the absence of MMP13, we explored the possibility that the lack of MMP13 activity was compensated for by changes in expression of other MMPs or in their inhibitors, the TIMPs.
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These further confirmed that expressions of the genes in the chitin biosynthesis pathway are affected by changes in expressions of trehalase genes.
From microarray data, it is clear that the mutant phenotype was caused by change in expression of flower development related genes and that both mutants show similar expression profiles (Figure 4).
Genes were grouped by change in expression, then subgrouped into Hairy bound or unbound, and finally ranked by fold change in gene expression.
For TAF knockdown experiments, human 18S rRNA (RN18S1) was used as the endogenous control, because its expression should not be affected by changes in TAF expression.
A number of ERRα target genes were regulated similarly including medium chain acylcoenzyme A dehydrogenase (MCAD), although the expression of ERRα is altered by changes in RIP140 expression.
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Justyna Jupowicz-Kozak
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