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Insoluble debris was removed by centrifugation in a table centrifuge at 20000 g at 4°C for 20 minutes.
Slides were dried by centrifugation in a mini-centrifuge.
The insoluble material was removed by centrifugation in a micro-centrifuge.
Plasma was then separated from red cells by centrifugation in a refrigerated centrifuge at 4 °C and transferred to a separate tube.
The yeast were pelleted by centrifugation in a fixed-angle centrifuge at 4500 g for 5 minutes at 4°C.
Prior ultrafiltration some ICF samples were heat cleared at 80°C for 10 min in a heating block and the heat sensitive proteins removed by centrifugation in a table top centrifuge (16,000 × g, 10 min).
SupT1 cells, grown in RPMI supplemented with 10% FCS, Pen-Srep, L-Glutamine, was treated with 5µg/mL Polybrene (Hexadimethrene Bromide, Sigma) and infected with viral stocks by centrifugation in a hanging bucket rotors centrifuge (Becton Dickinson) at 1500RPM, for 120 minutes at 32°C.
Briefly, 2 × 10 cells were scraped into 50 μl of lysis buffer (50 nM Tris HCl pH 8, 1% NP-40, 150 mM NaCl, 100 μg/ml leupeptin and 5 mM sodium vanadate), and supernatants were cleared by centrifugation in a standard table-top centrifuge at maximum speed at 4°C.
Lysate was clarified by centrifugation in a Sorvall RC-5B superspeed centrifuge at 38,724 × g for 1 hr and subsequently precipitated with 0.78 M (NH4 2SO4 for 30 min on ice.
Cell debris was then pelleted by centrifugation in a JA20 rotor in a Beckman J2-21M/E centrifuge at 31400 xg (20000 rpm) at 4ºC for 20 minutes.
Cells were harvested by centrifugation in a JA10 rotor in a Beckman J2-21M/E centrifuge at 2740 xg (5000 rpm) at 4ºC for 15 minutes.
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