Sentence examples for by cell surface staining from inspiring English sources

For the intracellular Ckb staining, the cells were firstly incubated with anti-Ckb, and then PE-conjugated anti-rabbit IgG, followed by cell surface staining.

This was followed by cell surface staining for CD4 to identify cells from either the CD4+ or CD4− lineage and anti-Vβ11 to exclude any contaminating non-NKT cells.

Donor contribution in peripheral blood, mature bone marrow lineage, bone marrow stem cell and progenitor cell compartment, and spleen were measured by cell surface staining for the CD45.1 and CD45.2 markers as well as genomic DNA analysis of sorted populations.

However, by cell surface staining, they were almost all negative for SSEA1 and mostly positive for SSEA3, TRA-1-60, and TRA-1-81 consistent with the phenotype of hESC (Fig. 4B).

Cell surface expression of IL-10R1, IL-10R2, M-CSF receptor (M-CSFR), TNFR1, and TNFR2 was analysed by cell surface staining and flow cytometry as described previously [ 22, 23].

For analysis of intracellular cytokines, cells were restimulated for 5 hr with 1 µg/ml MHC class I restricted peptides in the presence of 1 µg/ml brefeldin A, followed by cell surface staining and intracellular staining for IFN-γ.

Cells were stained with anti-CD16/32 anti-CD16/32en), followed BD Pharmingence staining with followedjugated anti-CD4 (GK 1.5), PE-anti-CD8 (clone 53-6.7), or APC-by20 (cell-surface2).

In the generated stable HeLa-CD95 cell lines, CD95 appeared to be transported to the cell surface as monitored by flow cytometry cell surface staining (Figure S3B).

Importantly, the introduction of the mutations at the glycosylation sites did not block transport of CD95 to the cell surface as monitored by flow cytometry cell surface staining (Figure S3A).

The presence of PS at the outer membrane leaflet, as determined by a specific cell surface staining using the Ca2+-dependent phospholipid binding protein Annexin-V [30] serves as an early marker for apoptotic cell death [31].

By means of cell surface staining for FACS analysis they are often defined as CD4+CD25+CD127lowCD3+ T-cells.