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Isolation of HSCs or LSCs by cell surface markers is better suited for subsequent functional studies such as in vivo transplantation since the Hoechst dye is toxic to the cells and therefore might impair their physiological functions.
hMSCs were isolated and identified by cell surface markers, then, cultured in Minimum Essential Medium Alpha Medium (α-MEM) supplemented with 10% FBS, 2 mM L-Glutamine, 100 U/mL penicillin and 100 µg/mL streptomycin, and incubated in a humidified atmosphere of 5% CO2 at 37°C.
Having observed that SALL4 was enriched in HS/PCs that were identified by cell surface markers, we planned to evaluate the expression of this stem cell factor in SP and non-SP cells in various blood cancer cell lines (HL-60, NB4, KG1a and RPMI8226 cells), as well as investigate the role of SALL4 in drug resistance and cancer stem cells as identified by SP studies.
ASCs can be identified by cell surface markers that distinguish different B cell populations.
However, a maturation status of a macrophage might be more complex than just defined by cell surface markers or morphology.
Here we used a FACS-based technique that allowed us to identify producer cells by cell surface markers and cytokine production simultaneously (13).
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As demonstrated here and elsewhere [18], [19], [40], several distinct subsets of the RDC (as defined by cell surface marker expression) can be identified in the normal/non-inflamed murine respiratory tract.
The isolation and characterization of human UCB-MSCs were performed by cell surface marker analysis and multilineage differentiation in our previous report.
After validation of the reporter system, we set out to characterize the cellular identity of PI-MECs by FACS analysis by using cell surface markers for the various epithelial populations of the mammary gland.
Specific cell subsets defined by their cell surface markers were sorted by ARIA cell sorter (BD Biosciences).
They are defined by different cell surface markers and characterized by specific phenotypic traits.
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